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Lakshmana Reddy, D. C.
- Comparison of Traditional Grow-Out Test and DNA-Based PCR Assay to Estimate F1 Hybrid Purity in Cauliflower
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PDF Views:75
Authors
Affiliations
1 Division of Biotechnology, Centre for Post-Graduate Studies, Jain University, Bengaluru 560 011, IN
2 Division of Biotechnology, Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Hesaraghatta, Bengaluru 560 089, IN
3 Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, GKVK, Bengaluru 560 065, IN
4 Division of Floriculture and Medicinal Crops, Indian Institute of Horticultural Research, Bengaluru 560 089, IN
1 Division of Biotechnology, Centre for Post-Graduate Studies, Jain University, Bengaluru 560 011, IN
2 Division of Biotechnology, Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Hesaraghatta, Bengaluru 560 089, IN
3 Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, GKVK, Bengaluru 560 065, IN
4 Division of Floriculture and Medicinal Crops, Indian Institute of Horticultural Research, Bengaluru 560 089, IN
Source
Current Science, Vol 115, No 11 (2018), Pagination: 2095-2102Abstract
Cauliflower (Brassica oleracea) is a cool-season crop belonging to the Brassicaceae family. Use of morphological differences between true-to-types and off-types in grow-out test (GOT) is the basic method for hybrid purity analysis. Traditional GOT is costly, tedious, time consuming and environment sensitive. To increase the speed and accuracy of genetic purity testing of hybrids, recent advances in DNA markers have shown promise. In the present study, the purity of cauliflower hybrid (NBH Tania-815) was assessed by traditional GOT and advanced molecular marker systems. The experiment was carried out by mixing 95% F1 hybrids with 5% female parents, individually in the sample sets of 400, 300, 200, 100, 80 and 40. For each sample size, PCR-based assay and GOT were carried out to check the hybrid purity. In the PCR-based assay, 220 pairs of SSR markers were screened, with 32 markers showing parental polymorphism including one codominant marker (BrgMS565). The purity level was determined by the co-dominant marker. A minimum sample size of 100 was standardized to confirm the hybrid purity as it showed the same result with that of higher sample sizes (200, 300 and 400). Hence, it is proposed that molecular marker-based hybrid purity assessment may serve as an effective substitute to traditional GOT.Keywords
Cauliflower, Grow-Out Test, Hybrid Purity, PCR Assay.Full Text
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- Isolation and Characterization of NBS-Encoding Disease Resistance Gene Analogs in Watermelon against Fusarium Wilt
Abstract Views :258 |
PDF Views:75
Authors
Affiliations
1 Division of Biotechnology, Indian Institute of Horticultural Research, Bengaluru 560 089, IN
2 Division of Vegetable Crops, Indian Institute of Horticultural Research, Bengaluru 560 089, IN
1 Division of Biotechnology, Indian Institute of Horticultural Research, Bengaluru 560 089, IN
2 Division of Vegetable Crops, Indian Institute of Horticultural Research, Bengaluru 560 089, IN
Source
Current Science, Vol 117, No 4 (2019), Pagination: 617-626Abstract
Fusarium wilt (Fusarium oxysporum f. sp. niveum) in watermelon is one of the deadliest diseases around the globe, and availability of disease-resistant varieties is moderate. Disease management utilizing resistance genes (R-genes)/resistance gene analogs (RGAs) has proven to be a promising and successful approach. In the present study, six watermelon RGAs were isolated from wild, fusarium wilt resistant genotype IIHR-82 (Citrullus lanatus var. citroides) using degenerate primers that identify nucleotide binding site-leucine-rich repeat (NBS–LRR) regions. Multiple sequence alignment of these RGAs identified the characteristic NBS– LRR motif, and BLASTp search revealed similarity of these RGAs with other pathogenesis-related proteins. Phylogeny and motif analysis revealed genetic diversity of RGAs within those isolated from watermelon and with other plant R-genes. The watermelon RGAs isolated in this study contained both TIR–NBS–LRR (TNL) and non-TIR–NBS–LRR (CNL) classes of Rgenes. Protein secondary structure prediction of these watermelon RGAs revealed the composition of proteins, including α -helix, β -strand, disordered region and other template-related information. Watermelon RGAs identified in the present study will help in the development of RGA-based markers for resistance to fusarium wilt of watermelon.Keywords
Disease Management, Fusarium Wilt, Resistance Genes, Watermelon.Full Text
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